Journal: bioRxiv

Article Title: Crosstalk between Stromal cells and Macrophages Shapes Host Immunity to Mycobacteria

doi: 10.64898/2026.02.05.704052

Figure Lengend Snippet: A. Graphical abstract of SC reduction in Pdgfrα creER :R26 DTA/+ mice with tamoxifen. B. Depicted are means ± SEM of absolute numbers of Pdgfrα + SC per omentum and per mg in Pdgfrα creER :R26 +/+ control (n=4) and infected mice (n=4) and Pdgfrα creER :R26 DTA/+ control (n=6) and infected mice (n=7) in 5 independent experiments (two-way ANOVA with Sidak multiple comparisons test). One Pdgfrα creER :R26 +/+ mouse with insufficient infection (no CFU counts) was removed (only stated once for complete ). C. Depicted are mean ± SEM of omentum weights in Pdgfrα creER :R26 +/+ control (n=4) and infected mice (n=5) and Pdgfrα creER :R26 DTA/+ control (n=6) and infected mice (n=7) in 5 independent experiments (two-way ANOVA with Sidak multiple comparisons test). D. Shown are means ± SEM of absolute omental macrophages numbers per mg, (two-way ANOVA with Sidak multiple comparisons test), (compare 6B) E. Shown are means ± SEM of Tim4 + macrophages in omentum per mg (two-way ANOVA with Sidak multiple comparisons test), (compare 6B). F. Shown are means ± SEM of Tim4 − Ly6C − macrophages in omentum per mg (two-way ANOVA with Sidak multiple comparisons test), (compare 6B). G. Shown are means ± SEM of Tim4 − Ly6C + macrophages in omentum per mg (two-way ANOVA with Sidak multiple comparisons test), (compare 6B). H. Depicted are means ± SEM of the relation of Ly6C + Tim4 − and Ly6C − Tim4 − macrophage subsets in omentum (compare 6B). I. Depicted are means ± SEM of M-CSF serum levels. J. NicheNet analysis from single cell sequencing dataset comparing the ligand expression on stromal cells to differentially expressed genes (receptors) between infected and control macrophages. K. In vitro co-culture of iMoP isolated from bone marrow of WT mice +/− stromal cells isolated from omentum, +/− M-CSF. Surface marker expression was analyzed by FACS 24 hours after plating. Depicted is mean ± SEM of 4-5 replicates in 2 independent experiments (two-way ANOVA with Sidak multiple comparisons test between conditions +/−SC). L. NicheNet analysis from a published single cell sequencing dataset of macaques comparing the ligand expression on fibroblasts to differentially expressed genes (receptors) between macrophages in high and low burden granulomas. M. Clustering and annotation of macrophages, inflammatory monocytes and stromal cells in a published human single cell sequencing data set of granulomas with high and low burden . N. NicheNet analysis from comparing the ligand expression on stromal cells to differentially expressed genes (receptors) between macrophages of high and low burden granulomas.

Article Snippet: Pdgfrα CreERT mice (B6.129S-Pdgfratm1.1(cre/ERT2)Blh/J) and β-Actin-GFP mice (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) were purchased from Jackson Laboratories (USA).

Techniques: Control, Infection, Single Cell, Sequencing, Expressing, In Vitro, Co-Culture Assay, Isolation, Marker

Journal: Cell

Article Title: Renal PIEZO2 is an essential regulator of renin

doi: 10.1016/j.cell.2025.11.013

Figure Lengend Snippet: A. Representative Sun1-sfGFP fl/fl ; Pdgfrb CreERT2 mouse kidney. B. UMAP projection of Sun1-sfGFP fl/fl ; Pdgfrb CreERT2 snRNA-seq. C . Multidimensional dot plot of cell type-specific markers used to identify clusters corresponding to distinct Pdgfrb + kidney cell populations in D . Violin plot of Piezo2 . E. Feature plot of Piezo2 expression in UMAP space. F . Violin plot of Piezo1 . G. Feature plot of Piezo1 expression in UMAP space. See also – .

Article Snippet: Pdgfrb CreERT2 ( B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J , Jackson Laboratories 029684) mice were crossed to CAG-Sun1-sfGFP (B6;129-Gt(ROSA)26Sor tm5(CAG-Sun1/sfGFP)Nat /J, Jackson Laboratories 021039) mice to generate the Pdgfrb CreERT2 -INTACT (Isolation of Nuclei TAgged in specific Cell Types) mice used for snRNA-Seq experiments., Two male Pdgfrb CreERT2 -INTACT mice were i.p. injected with 1 mg tamoxifen daily for five days to induce the sfGFP expression.

Techniques: Expressing

Journal: Cell

Article Title: Renal PIEZO2 is an essential regulator of renin

doi: 10.1016/j.cell.2025.11.013

Figure Lengend Snippet: A. Plasma renin levels (Mann–Whitney: ** p = 0.0019, U = 48; n = 20 Pdgfrb WT and 13 Pdgfrb CreERT2 mice). B. Plasma aldosterone levels (Mann–Whitney: p = 0.8653, U = 84; n = 11 Pdgfrb WT and 16 Pdgfrb CreERT2 mice). C. Plasma Ang II levels (Mann–Whitney: p = 0.1932, U = 21; n = 7 Pdgfrb WT and 10 Pdgfrb CreERT2 mice). D. Sectioned kidney representative of n = 4 fields-of-view (two each on two slides) from N = 3 mice from Piezo2 fl/fl ; Pdgfrb WT mouse (scale = 100 μm). E. Sectioned kidney representative of n = 4 fields-of-view (two each on two slides) from N = 3 mice from Piezo2 fl/fl ; Pdgfrb CreERT2 mouse (scale = 100 μm). F. JG index (Welch’s t-test: * p = 0.0215, t = 3.847; df = 3.673; N = 3 Pdgfrb WT and 3 Pdgfrb CreERT2 kidneys from 3 mice each). G. Systemic blood pressure (systolic/SAP; diastolic/DAP; and mean arterial pressure/MAP; two-tailed nested t -tests (left to right): ** p SAP = 0.0013, t = 3.899, d.f. = 16; ** p DAP = 0.0056, t = 3.197, d.f. = 16; ** p MAP = 0.0027, t = 3.546, d.f. = 16; n = 10 Pdgfrb WT and 8 Pdgfrb CreERT2 mice). H. Plasma renin levels (Mann–Whitney: ** p = 0.0047, U = 3; n = 6 Ren WT and 8 Ren Cre mice). I. Systemic blood pressure (two-tailed nested t -tests (left to right): * p SAP = 0.0147, t = 2.716, d.f. = 17; ** p DAP = 0.0097, t = 2.913, d.f. = 17; ** p MAP = 0.0060, t = 3.137, d.f. = 17; n = 9 Ren WT and 10 Ren Cre mice). Each experiment was performed on at least two independent cohorts of mice, and error bars represent mean ± s.e.m. See also and – .

Article Snippet: Pdgfrb CreERT2 ( B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J , Jackson Laboratories 029684) mice were crossed to CAG-Sun1-sfGFP (B6;129-Gt(ROSA)26Sor tm5(CAG-Sun1/sfGFP)Nat /J, Jackson Laboratories 021039) mice to generate the Pdgfrb CreERT2 -INTACT (Isolation of Nuclei TAgged in specific Cell Types) mice used for snRNA-Seq experiments., Two male Pdgfrb CreERT2 -INTACT mice were i.p. injected with 1 mg tamoxifen daily for five days to induce the sfGFP expression.

Techniques: Clinical Proteomics, MANN-WHITNEY, Two Tailed Test

Journal: Cell

Article Title: Renal PIEZO2 is an essential regulator of renin

doi: 10.1016/j.cell.2025.11.013

Figure Lengend Snippet: A. GFR measurement in mice (created with BioRender.com ). B. GFR (Mann–Whitney: **** p < 0.0001, U = 7; n = 12 Pdgfrb WT and 12 Pdgfrb CreERT2 mice). C. GFR (Mann–Whitney: **** p < 0.0001, U = 1; n = 12 Ren WT and 10 Ren Cre mice. D. Captopril experiment (created with BioRender.com ). E. GFR after captopril (Mann– Whitney: p = 0.4470, U = 35; n = 10 Pdgfrb WT and 9 Pdgfrb CreERT2 mice). F. Plasma renin levels after captopril (Mann–Whitney: p = 0.8820, U = 47; n = 11 Pdgfrb WT and 9 Pdgfrb CreERT2 mice). G. A779 MAS blockade experiment (created with BioRender.com ). H. GFR before (pre-A779) and after (post-A779) treatment with A779 (two-way ANOVA: **** p interaction < 0.0001, F(1,6) = 248.9; Sidak’s multiple comparisons: **** p pre-A779 < 0.0001, p post-A779 = 0.7724; n = 4 Pdgfrb WT and 4 Pdgfrb CreERT2 mice). I. GFR before (pre-A779) and after (post-A779) treatment with A779 (two-way ANOVA: **** p interaction < 0.0001, F(1,15) = 28.69; Sidak’s multiple comparisons: **** p pre-A779 < 0.0001, p post-A779 = 0.6662; n = 8 Ren WT and 9 Ren Cre mice). Each experiment was performed on at least two cohorts of mice, and error bars represent mean ± s.e.m. See also .

Article Snippet: Pdgfrb CreERT2 ( B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J , Jackson Laboratories 029684) mice were crossed to CAG-Sun1-sfGFP (B6;129-Gt(ROSA)26Sor tm5(CAG-Sun1/sfGFP)Nat /J, Jackson Laboratories 021039) mice to generate the Pdgfrb CreERT2 -INTACT (Isolation of Nuclei TAgged in specific Cell Types) mice used for snRNA-Seq experiments., Two male Pdgfrb CreERT2 -INTACT mice were i.p. injected with 1 mg tamoxifen daily for five days to induce the sfGFP expression.

Techniques: MANN-WHITNEY, Clinical Proteomics

Journal: Cell

Article Title: Renal PIEZO2 is an essential regulator of renin

doi: 10.1016/j.cell.2025.11.013

Figure Lengend Snippet: A. Plasma renin levels after saline (Mann–Whitney: *** p = 0.0007, U = 0; n = 6 Pdgfrb WT and 8 Pdgfrb CreERT2 mice). B. Plasma aldosterone levels after saline (Mann–Whitney: p = 0.1419, U = 12; n = 6 Pdgfrb WT and 8 Pdgfrb CreERT2 mice). C. Plasma renin levels (two-way ANOVA: **** p interaction < 0.0001, F(1,26) = 29.45434; Uncorrected Fisher’s LSD (left to right): **** p < 0.0001, **** p < 0.0001, p = 0.6164; n = 8 Pdgfrb WT control, 6 Pdgfrb WT high sodium, 8 Pdgfrb CreERT2 control, and 8 Pdgfrb CreERT2 high sodium mice). D. Plasma aldosterone levels in mice from C (two-way ANOVA: *** p genotype = 0.0005, F(1,26) = 15.83434; Uncorrected Fisher’s LSD (left to right): p = 0.1, ** p = 0.0092, *** p = 0.0002; n = 8 Pdgfrb WT control, 6 Pdgfrb WT high sodium, 8 Pdgfrb CreERT2 control, and 8 Pdgfrb CreERT2 high sodium mice). E. Plasma renin levels (two-way ANOVA: ** p interaction = 0.0030, F(1,28) = 10.55225; Uncorrected Fisher’s LSD (left to right): **** p < 0.0001, **** p < 0.0001, p = 0.8084, p = 0.4351; n = 8 Pdgfrb WT control, 8 Pdgfrb WT sodium deficient, 8 Pdgfrb CreERT2 control, and 8 Pdgfrb CreERT2 sodium deficient mice). F. Plasma aldosterone from mice in E (two-way ANOVA: * p interaction = 0.0305, F(1,28) = 5.194771; Uncorrected Fisher’s LSD (left to right): **** p < 0.0001, p = 0.7840, ** p = 0.0016, **** p < 0.0001; n = 8 Pdgfrb WT control, 8 Pdgfrb WT sodium deficient, 8 Pdgfrb CreERT2 control, and 8 Pdgfrb CreERT2 sodium deficient mice). Each experiment was performed on at least two independent cohorts of mice, and error bars represent mean ± s.e.m. Data from C and E were subjected to a log-transform prior to statistical analysis. Data from control mice are replotted in C-D vs. E-F with statistics run separately.

Article Snippet: Pdgfrb CreERT2 ( B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J , Jackson Laboratories 029684) mice were crossed to CAG-Sun1-sfGFP (B6;129-Gt(ROSA)26Sor tm5(CAG-Sun1/sfGFP)Nat /J, Jackson Laboratories 021039) mice to generate the Pdgfrb CreERT2 -INTACT (Isolation of Nuclei TAgged in specific Cell Types) mice used for snRNA-Seq experiments., Two male Pdgfrb CreERT2 -INTACT mice were i.p. injected with 1 mg tamoxifen daily for five days to induce the sfGFP expression.

Techniques: Clinical Proteomics, Saline, MANN-WHITNEY, Control

Journal: Cell

Article Title: Renal PIEZO2 is an essential regulator of renin

doi: 10.1016/j.cell.2025.11.013

Figure Lengend Snippet: A. PEG model of hypovolemia (created with BioRender.com ). B. Plasma renin levels (Mann–Whitney: *** p = 0.0003, U = 53; n = 17 Pdgfrb WT and 19 Pdgfrb CreERT2 mice). C. Plasma aldosterone levels (Mann–Whitney: * p = 0.0257, U = 48; n = 12 Pdgfrb WT and 16 Pdgfrb CreERT2 mice). D. Plasma Ang II levels (Mann–Whitney: ** p = 0.0043, U = 0; n = 5 Pdgfrb WT and 6 Pdgfrb CreERT2 mice). E. Plasma renin levels (Mann–Whitney: ** p = 0.0025, U =18; n = 13 Ren WT and 10 Ren Cre mice). F. Plasma aldosterone levels (Mann–Whitney: **** p < 0.0001, U =2; n = 15 Ren WT and 10 Ren Cre mice). G. Plasma renin levels (Mann–Whitney: * p = 0.0360, U =14; n = 8 FoxD1 WT and 9 FoxD1 Cre mice). H. Plasma aldosterone levels (Mann–Whitney: * p = 0.0418, U =12; n = 7 FoxD1 WT and 9 FoxD1 Cre mice). Each experiment was performed on at least two independent cohorts of mice, and error bars represent mean ± s.e.m. See also and .

Article Snippet: Pdgfrb CreERT2 ( B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J , Jackson Laboratories 029684) mice were crossed to CAG-Sun1-sfGFP (B6;129-Gt(ROSA)26Sor tm5(CAG-Sun1/sfGFP)Nat /J, Jackson Laboratories 021039) mice to generate the Pdgfrb CreERT2 -INTACT (Isolation of Nuclei TAgged in specific Cell Types) mice used for snRNA-Seq experiments., Two male Pdgfrb CreERT2 -INTACT mice were i.p. injected with 1 mg tamoxifen daily for five days to induce the sfGFP expression.

Techniques: Clinical Proteomics, MANN-WHITNEY